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An oriented self-assembly biosensor with built-in error-checking for precise midkine detection in cancer diagnosis and prognosis evaluation 期刊论文

编号：

1F15C52BBD0A9F5636B3523074F65996
作者：

Jiao, Jun#*^[1]Yin, Mengai#^[1]Wang, Zhijie^[1];Hu, Bingxin^[1];Chi, Jiadong(池嘉栋)^[2]Lu, Lina^[1];Dai, Fuju^[1];Xue, Lan^[1];Wang, Tong^[1];Wang, Xiangrui^[1];Zhao, Jie^[4];Zhao, Li*^[3]Chen, Qiang*^[1]
语种：

英文
期刊：

BIOSENSORS & BIOELECTRONICS ISSN：0956-5663 2025 年 268 卷 ; JAN 15
收录：
关键词：

Midkine detection Cancer diagnosis and prognosis evaluation Biosynthesized quantum dots Oriented immobilization Built-in error-checking
摘要：

Midkine (MDK) is a neurotrophic growth factor highly expressed during embryogenesis, currently recognized as a multifaceted factor in cancer progression and drug resistance. MDK has demonstrated greater accuracy than existing biomarkers. Serum MDK is a valuable indicator for the non-invasive early detection of tumors. It dynamically changes following surgical tumor excision and prior to recurrence, facilitating prognosis and treatment response evaluation. However, existing methods struggle to achieve the sensitivity required for clinical applications. Herein, we developed a triple-mode biosensor with oriented self-construction and built-in errorchecking for rapid, sensitive, and convenient MDK detection. The sensor construction adhered to the principle of achieving oriented and strong covalent connections to ensure high sensitivity. Biosynthesized quantum dots (BQDs) were introduced to orient antibodies, enhancing the exploration of active binding sites and significantly increasing antibody-capturing ability. To further enhance sensitivity and signal amplification, Au@Pt nanorodsAb2 (MF-Probe) were used as multifunctional probes, incorporating an error-checking mechanism to minimize false results. Detection was feasible using an electrochemical workstation, a microplate reader, and even a mobile phone. The sensor exhibited a wide linear range from 5 fg/mL to 100 ng/mL and a low limit of detection (LOD) of 1.620 fg/mL. It accurately distinguished MDK levels in the serum of healthy donors and cancer patients. Compared to existing ELISA kits, it exhibited a lower LOD and a more sensitive response to trace MDK, suggesting it is a promising tool for cancer diagnosis and prognostic evaluation.
推荐引用方式
GB/T 7714：

Jiao Jun,Yin Mengai,Wang Zhijie, et al. An oriented self-assembly biosensor with built-in error-checking for precise midkine detection in cancer diagnosis and prognosis evaluation [J].BIOSENSORS & BIOELECTRONICS,2025,268.
APA：

Jiao Jun,Yin Mengai,Wang Zhijie,Hu Bingxin,&Chen Qiang.(2025).An oriented self-assembly biosensor with built-in error-checking for precise midkine detection in cancer diagnosis and prognosis evaluation .BIOSENSORS & BIOELECTRONICS,268.
MLA：

Jiao Jun, et al. "An oriented self-assembly biosensor with built-in error-checking for precise midkine detection in cancer diagnosis and prognosis evaluation" .BIOSENSORS & BIOELECTRONICS 268(2025).
入库时间：

12/2/2024 9:25:08 PM
更新时间：

12/23/2024 9:41:48 PM
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