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Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system 期刊论文

编号：

2F298B67FDD72B2A5894C4DF716296C7
作者：

Yang, Jie#^[1,2]Wang, Tongyao^[1,2];Liu, Zhikun^[1,2];Wu, Wenqi^[1,2,3];Sun, Yangyue^[1,2];Zhan, Yancheng^[1,2];Zhang, Shuqin^[1,2];Chen, Hong^[1];Liu, Bin^[1];Yue, Caidie^[1];Yin, Zhenning^[1];Shan, Zhengda^[1,4];Li, Xuzichao^[1];Li, Zhuang^[5];Yuan, Zhiyong(袁智勇)^[4]Yin, Hang^[1,6];Zhang, Heng*^[1,2,4]
语种：

英文
期刊：

MOLECULAR CELL ISSN：1097-2765 2026 年 86 卷 7 期 (1217 - 1229) ; APR 2
收录：
摘要：

The RNA-directed programmable nuclease systems, exemplified by the CRISPR-Cas system, have been widely used in genome editing. In contrast to the single-spacer configuration of CRISPR RNA (crRNA), the guide RNA (tigRNA) of the tandem interspaced guide RNA (TIGR) system features a dual-spacer arrangement, thereby directing the TIGR-associated (Tas) protein to engage both strands of the target double-stranded DNA (dsDNA). Here, we determine six cryo-electron microscopy structures of the Salicola phage TIGR-TasH complex. The central coiled-coil region of TasH mediates dimerization, while the C-terminal nucleolar protein (Nop) domain is able to autonomously process precursor tigRNA. Upon target binding, the dynamic N-terminal HNH nuclease domain is recruited for cleavage through a beta-hairpin, which also determines the target preference. More interestingly, the conserved box C motif of tigRNA stabilizes this beta-hairpin in an adenine-specific manner, enabling us to rationally design a guide RNA-defined nickase, distinct from conventional protein-based nickase strategies used in genome editing.
推荐引用方式
GB/T 7714：

Yang Jie,Wang Tongyao,Liu Zhikun, et al. Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system [J].MOLECULAR CELL,2026,86(7):1217-1229.
APA：

Yang Jie,Wang Tongyao,Liu Zhikun,Wu Wenqi,&Zhang Heng.(2026).Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system .MOLECULAR CELL,86(7):1217-1229.
MLA：

Yang Jie, et al. "Molecular basis for dual-spacer-guided target cleavage by the TIGR-TasH system" .MOLECULAR CELL 86,7(2026):1217-1229.
入库时间：

4/30/2026 9:35:03 PM
更新时间：

4/30/2026 9:35:03 PM

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