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A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis  期刊论文  

  • 编号:
    A63FD15312F4AE3AD87AAAE2DE29B08B
  • 作者:
    Bao, Kaiwen#[1]Ma, Yanhui#[1]Li, Yuan#[1]Shen, Xilin#[1]Zhao, Jiao#[1]Tian, Shanshan#[1]Zhang, Chunyong[1];Liang, Can[1];Zhao, Ziyan[1];Yang, Ying[2];Zhang, Kai[1];Yang, Na[3];Meng, Feilong[4];Hao, Jihui[1];Yang, Jie[1];Liu, Tao[5];Yao, Zhi[1];Ai, Ding*[1]Shi, Lei*[1]
  • 语种:
    英文
  • 期刊:
    MOLECULAR CELL ISSN:1097-2765 2024 年 84 卷 7 期 ; APR 4
  • 收录:
  • 摘要:

    Appropriate DNA end synapsis, regulated by core components of the synaptic complex including KU70KU80, LIG4, XRCC4, and XLF, is central to non -homologous end joining (NHEJ) repair of chromatinized DNA double -strand breaks (DSBs). However, it remains enigmatic whether chromatin modifications can influence the formation of NHEJ synaptic complex at DNA ends, and if so, how this is achieved. Here, we report that the mitotic deacetylase complex (MiDAC) serves as a key regulator of DNA end synapsis during NHEJ repair in mammalian cells. Mechanistically, MiDAC removes combinatorial acetyl marks on histone H2A (H2AK5acK9ac) around DSB-proximal chromatin, suppressing hyperaccumulation of bromodomain-containing protein BRD4 that would otherwise undergo liquid -liquid phase separation with KU80 and prevent the proper installation of LIG4-XRCC4-XLF onto DSB ends. This study provides mechanistic insight into the control of NHEJ synaptic complex assembly by a specific chromatin signature and highlights the critical role of H2A hypoacetylation in restraining unscheduled compartmentalization of DNA repair machinery.

  • 推荐引用方式
    GB/T 7714:
    Bao Kaiwen,Ma Yanhui,Li Yuan, et al. A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis [J].MOLECULAR CELL,2024,84(7).
  • APA:
    Bao Kaiwen,Ma Yanhui,Li Yuan,Shen Xilin,&Shi Lei.(2024).A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis .MOLECULAR CELL,84(7).
  • MLA:
    Bao Kaiwen, et al. "A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis" .MOLECULAR CELL 84,7(2024).
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